WebScorpions PCR Primers. These assays use two PCR primers, one of which serves as a probe and contains a stem-loop structure with a 5' fluorescent reporter and 3' quencher. The loop of the Scorpions probe includes a … WebLabeling various targets with separate fluorescent colors allows you to visualize different …

Live cell staining fluorescent dyes guide Abcam

WebAug 6, 2024 · Covalently tagging proteins with chemical dyes has advantages over using fluorescent proteins (FPs), including the following: access to a wide range of fluorescent dyes, which typically have higher quantum yield and photostability than FPs; initiation of fluorescence when the dye is conjugated to the target protein, providing temporal control … WebCy3 dye. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids. The Cy3 fluorophore is also the basis for the lipophilic neuronal and long ... team penning videos

Live cell staining fluorescent dyes guide Abcam

WebOct 13, 2024 · An alternative option is to use a self-labeling tag like Halo tag and an infrared dye; these dyes, such as Cy5 or Alexa 647, are substantially brighter than the infrared fluorescent proteins. Four-color imaging with this combination is relatively straightforward, as both the fluorescent proteins and relevant filter sets are readily available. WebOur fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, … WebActivated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, and enzyme assays. The Atto dyes are a series of fluorescent dyes that meet the critical needs of … ekologisk granola