Tmre flow cytometry protocol
WebAnalyze cells by flow cytometry. Alternatively, counterstain cells with compatible fluorescent dyes or antibodies as desired and then analyze. BD Pharmingen™ MitoStatus TMRE … WebThe MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCP for the induction of …
Tmre flow cytometry protocol
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WebFlow Cytometry Reagents ... TMRE (Tetramethylrhodamine ethyl ester, perchlorate) and TMRM are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation. ... Protocols. PI-70016. SDS. SDS-70016. Supporting Documents. Cellular Stains Brochure. WebNov 22, 2015 · Recently we showed that TMRE staining could be successfully used in flow cytometry (with 561 nm excitation laser) and loss of TMRE staining is tightly coordinated …
WebTo develop a standardized protocol for measuring MMP changes in individual primary lymphocytes. Materials and Methods Cell purification and labeling: T‐lymphocytes are … WebBD Pharmingen™ MitoStatus TMRE labeling of cells for fluorescent imaging 1. Stain cells in fresh, pre-warmed media with 20-200 nM BD Pharmingen™ MitoStatus TMRE. 2. Incubate samples for 15-30 minutes at 37°C protected from light. 3. Remove the staining solution and replace with pre-warmed DPBS. 4. Analyze cells by fluorescence imaging.
WebIn this ROS Protocols article, we described the flow cytometric protocol involving the use of various concentrations of MitoSOX (1, 2.5, 5 μM) for detecting mitochondrial ROS in … WebRather than providing a specific protocol, the following can serve as a general guide to the most frequently utilized options for apoptosis assessment. ... Among the probes available for MMP assessment are Rhodamine 123, CMX Rosamine, and TMRE. Choice of these or other similar dyes will depend upon experiment plan. ... Using flow cytometry to ...
WebThe double population characterized by their fluorescence signal, with TMRE and DIOC6(3), was a puzzling observation that led us to use flow cytometry coupled with imaging. This new technology allowed us first to verify the specificity of the fluorescence labelling, which was indeed uniquely localized to the middle piece of spermatozoa, with ...
WebTMRM and TMRE signals can be detected in live cells or isolate mitochondria with traditional and high content fluorescence microscopy, microplates, or by flow cytometry (Figure 2). … law online formWebProtocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging. ... Flow cytometry histogram of TMRE for CD48-LSKCD150 + HSCs treated with CCCP (100 μM, red), or oligomycin (2 μg/mL, blue), or untreated (DMSO, 10 min, black). Depolarized and hyperpolarized TMRE gates ... karate in high pointWebNov 22, 2015 · 3.2 TMRE and Caspase 3/7 Staining 1. Add TMRE from stock solution at a final concentration 50–100 nM. Typical stock solution is 50–100 μM in DMSO. 2. Add caspase 3/7 reagent—one drop per 0.5 ml is recommended, proper concentration could be titrated. 3. Place vial with cells into CO 2 incubator (37 °C) for 30 min. 3.3 Application of … law online free coursesWebDec 16, 2024 · The protocol below describes a method that fractionates phenotypically defined highly potent (mouse and human) HSCs based on MMP levels. ... Flow cytometry histogram of TMRE for CD48-LSKCD150 + HSCs treated with CCCP (100 μM, red), or oligomycin (2 μg/mL, blue), or untreated (DMSO, 10 min, black). Depolarized and … karate in high wycombeWebNational Center for Biotechnology Information karate in forney txWebsolution. Each vial includes enough TMRE for five 96-well plates (0.1 µl/well) or 50 flow cytometry assays (10 µl/assay). Aliquot if desired and store at -20°C. Dilute TMRE to 1:500 with full cell culture medium to make 10X TMRE Labeling Solution. 2 µM is used in this protocol; 0.1 to 10 µM is recommended based on different cell lines. 3. karate in hornchurchWebNov 18, 2015 · Add 100-200nM of TMRE to your culture medium, incubate the cells in the medium at 37C for 10min. You should light up their mitochondria. If your signal is weak, you may increase TMRE to 300mM.... law online programs