WebAsymmetric peaks: Tailing. Tailing peaks can be caused by an underpacked column, that is, a column packed at a very low pressure or flow rate. Ensure the resin is evenly packed. Tailing peaks can also be explained by uneven sample application or interactions with the column. Asymmetric peaks: Fronting Web15 Oct 2024 · In addition to filters, guard columns can prevent contaminants from making their way into the analytical column, but blocked or defective guard columns can also be a source of peak tailing. Before you go to wash your column back and forth, you can rule this out by removing the guard column and running a standard to see if the problem persists. 5.
Factors Affecting Resolution in HPLC - Sigma-Aldrich
Web17 Aug 2024 · Common causes of peak tailing and/or low resolution include (but are not limited to): Presence of Teflon (PTFE) in the sample flow path. Teflon is very hydrophobic, … WebPossible Cause: Column and Inlet Maintenance. Sudden issues with tailing indicates column contamination, possibly caused by a recent dirty sample. Using sample prep, such as SPE, … st mary redcliffe car park
Column Overloading - An issue of some sensitivity
Web10 Nov 2024 · Tailing or wider peaks, higher background signal, carry over, or ghost peaks can be a sign of a contaminated column. The use of low-quality solvents can also be the … Web1 Mar 2009 · A number of problems can cause volumetric peak tailing in GC. Installing a fused-silica column with the tip of the column too low in a split–splitless inlet can create tailing peaks because the full carrier gas split flow cannot reach the column entrance and sweep residual sample away. WebTailing peaks are usually created through turbulence within the hydraulic path of the carrier gas as it passes through the system. They may also be caused by unswept volumes … st mary recreation center