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Pef6/v5-his b

WebB I I Pme Comments for pEF6/V5-His A 5818 base pairs EF-1α promoter: bases 474-1651 T7 promoter/priming site: bases 1668-1687 Multiple cloning site: bases 1713-1804 V5 … WebThe pEF6/V5-His-TOPO® expression vector and the monoclonal mouse anti-V5 antibody were purchased from Invitrogen. Genomic DNA isolated from peripheral blood lymphocytes of healthy European-, African-, Chinese-, and Hispanic-American volunteers was purchased from Coriell Cell Repositories (Camden, NJ). An enzymatic deglycosylation kit was ...

Activin Type II Receptor Restoration in Colon Cancer Cells …

WebJun 2, 2014 · WT and mutant SOX11 cDNAs were PCR amplified and cloned into the pEF6/V5-His B mammalian expression vector (Life Technologies) using the In-Fusion PCR Cloning Kit (Clontech Laboratories), and also ... mild sloppy joe recipe https://needle-leafwedge.com

pEF6/V5-His A, B, and C - Gene Synthesis

WebpEF6/Myc-His To propagate and maintain the pEF6/Myc-His vectors, we recommend resuspending each vector in 20 μl sterile water to prepare a 1 μg/μl stock solution. Store the stock solution at -20°C. Use the stock solution to transform a recA, endA E. coli strain like TOP10F´, DH5αF´, or equivalent. WebAug 14, 2015 · zt321 pIZT/V5-His 载体 Invitrogen 昆虫细胞表达载体系统. zt322 pMIB v5-His B 载体 Invitrogen 昆虫细胞表达载体系统. zt323 pFBDM 载体 Invitrogen 昆虫细胞表达载体系统. zt324 pUCDM 载体 Invitrogen 昆虫细胞表达载体系统. zt325 pBADZ-His6Cre 载体 Invitrogen 昆虫细胞表达载体系统 Web[CANCER RESEARCH 64, 7690–7696, November 1, 2004] Advances in Brief Activin Type II Receptor Restoration in ACVR2-Deficient Colon Cancer Cells Induces Transforming Growth Factor- Response Pathway Genes mild soap for washing machine

pEF6/Myc-His A, B, and C - Thermo Fisher Scientific

Category:pEF6/Myc-His A, B, and C - Thermo Fisher Scientific

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Pef6/v5-his b

pEF6 V5-His B Sequence and Map - SnapGene

Web上海易汇生物科技有限公司，是一家集研发、销售为一体的高新技术生物企业，公司专注于生命科学和生物技术领域，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司目前已授权代理 LKT Laboratories，Epigent WebB I I Pme Comments for pEF6/V5-His A 5818 base pairs EF-1α promoter: bases 474-1651 T7 promoter/priming site: bases 1668-1687 Multiple cloning site: bases 1713-1804 V5 epitope: bases 1805-1846 6xHis tag: bases 1856-1873 BGH reverse priming site: bases 1896-1913

Pef6/v5-his b

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WebDensitometric analysis of (A) LRP6 phosphorylation and (B) total LRP6 content in cells treated with the indicated concentrations of Rspo2-2F, Rspo2-2F/Q70R and/or Wnt-3a for 1, 6 or 20 h prior to processing for immunoblotting. Cell lysates also were immunoblotted for HSP70 as a loading control. WebpEF6 V5-His B Sequence and Map Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5-His …

WebDec 8, 2006 · HEK-293T cells were transfected with pEF6/V5-His recombinant vector containing Lrmp (A), Casc1 (B), Ghiso (C), or Lmna-rs1 (D) full-length cDNAs, grown for 48 h, fixed with paraformaldehyde, and treated with anti-V5 monoclonal antibody followed by secondary antibody-Alexa Fluor 488 conjugate. WebpEF6 V5-His TOPO® TA Vector Kit pEF6/V5-His TOPO® TA Vector Kit, included in the MembranePro™ Functional Protein Expression Kit and also available separately (see page 29), contains the reagents listed below. Upon receipt, store the vector kit at –20°C. Reagent Composition Amount pEF6/V5-His-TOPO® vector 10 ng/µL plasmid DNA 20 µL

Webwere cloned separately into the pEF6 V5-His-TOPO mammalian expression vector (Invitrogen), and these plasmids were transfected into susceptible C3H He cells with Lipofectamine 2000 (Life Technologies, Grand Island, NY). Stable integrants were selected by using blasticidin S, and cells from individual foci were isolated with cloning rings and ... WebJan 15, 2013 · Full-length ADAMTS4 has a protumorigenic effect, whereas full-length E362A inhibits B16 melanoma tumor growth. (a) Expression of ADAMTS4 full-length proteins—WT and E362A mutant in conditioned medium of stably transfected B16 melanoma cell lines using pEF6/V5-His TOPO vector analyzed by immunoblotting with anti-V5 antibody. Blot …

Webs871 streptavidin, rhodamine b 1 mg 1627.5. s872 streptavidin, texas red（r 1 mg 1627.5. s888 streptavidin 5 mg 2096.5. s911 streptavidin, horseradish 1 mg 1627.5. s921 streptavidin, alkaline ph 0.5 ml 2612.4. s931 streptavidin, beta-galact 1 mg 2144.8. s951 streptavidin agarose sed 5 ml 2128. sa10001 streptavidin - hrp 50ul 1449. sa10002 ...

WebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO™ Cloning. The pEF6/V5-His … new year\u0027s eve potluckWebVector Data. Vivid colors pcDNA6.2/C-EmGFP/GW/CAT . Restriction; Sequence; Map; Vector Data. pENTR L1-pLac-lacZalpha-L4 new year\u0027s eve potawatomi casinoWebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia … new year\\u0027s eve potluck ideasWebiv Kit Contents and Storage Shipping and Storage pVAX1™ and pVAX1™/lacZ are shipped at room temperature. Upon receipt, store vectors at –20°C. Kit Contents 20 µg each of … new year\u0027s eve powerballWebApr 30, 2024 · PCR products were inserted into pEF6/V5-His B vector (Invitrogen) at EcoRV sites using In-Fusion HD Cloning Kit (TaKaRa) or NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA). The plasmids of dNlgns were modified by replacing V5-His tag with FLAG tag. Therefore, we obtained the following five plasmids: pEF6-dNlgn1-FLAG, … new year\u0027s eve potluck flyerWebContents 20 μg each pEF6/Myc-His A, B, and C, lyophilized 20 μg pEF6/Myc-His/lacZ, lyophilized Shipping/Storage Lyophilized plasmids are shipped at room temperature and … new year\u0027s eve potluck recipesWebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian český русский български العربية Unknown mild soap to use on dogs