2024 Jeg-3 cell line

Jeg-3 cell line

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Web13 mar 2024 · The culture of JEG-3 cells (a human choriocarcinoma cell line) has been described previously . To study the cytotoxic effect of α-solanine on JEG-3 cells, cells were cultured in 96-well plates overnight, followed by treatment with different concentrations of α-solanine (10, 20, 30, 40 and 50 μM) or vehicle (0.5% v/v DMSO) for 24 h. Web1 dic 2016 · Results: Our results showed that HTR-8/SVneo cell line contains two populations of cells suggesting the presence of trophoblast and stromal/mesenchymal cells. While all cells in BeWo, JEG-3 and ...

JEG-3 - German Collection of Microorganisms and Cell Cultures …

WebIn this study, we found that the human HLA-G-positive choriocarcinoma cell line JEG-3 upregulates CD4 + CD25 hi CD127 lo T cells, increases the expression of HLA-G + CD4 + and CD8 + T cells, and decreases the expression of ILT2 + on CD4 + T cells in resting PBMCs after six days of co-culture. Web23 set 2024 · The human chorionic cell line JEG-3 (HTB-36, ATCC) was provided from Fujian Maternity and Child Care Center, China. The breast cancer cell line MCF-7 (HTB-22, ATCC) was obtained from ATCC and used as a model of circulating tumor cells (CTCs). chest discomfort with hot flashes men

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WebFurthermore, retinoids are secreted from JEG-3, as shown by the activation of retinoic acid-responsive beta lacZ reporter cells grown in conditioned media. These results suggest … WebIt was postulated that 3D cell culture models more accurately reflect the complex tissue physiology and morphology in comparison to 2D cell monolayers. Currently, there is a shortage of well-characterized and easily maintainable high-throughput experimental models of the human placenta. Here, we characterized three different 3D cultures (e.g., … Web31 mar 2024 · The choriocarcinoma-derived JEG-3 cell-line (ATCC HTB-36, Manassas, VA, USA), was grown as recommended by ATCC: ... JEG-3 cells in 2.5% FBS released similar levels of hCG, hPL, and estradiol to JEG-3 cells in 10% FBS, and thus maintain the endocrine function of human placenta. chest discomfort with burping

ECACC General Cell Collection: 92120308 JEG3 - Culture Collections

Transthyretin regulates the migration and invasion of JEG-3 cells

WebFigure 10 The effect of overexpression or silencing of hsa-miR-29b-3p, hsa-miR-519c-3p and hsa-miR-520a-5p on cell proliferation in JEG-3 and JAR cells. Viability of JEG-3 and JAR cells transfected with hub DEM mimics or inhibitors measured by CCK-8 (A–D) and EdU assays (E and F). The percentage of Edu positive JEG-3 and JAR. Web15 gen 2024 · JEG-3 cells synthesize placenta-specific hormones, such as human chorionic gonadotrophin [1] and, in contrast to most other “trophoblastic” cell lines, … chest discomfort when sneezingWeb30 ott 2024 · The most commonly used cell line in studies on human placental transport is the choriocarcinoma cell line BeWo, ... The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells. Toxicol. Lett. 2013, 218, 200–206. [Google Scholar] Chen, Z.S ... good mpg sports cars

"Web31 mar 2024 · JEG-3 placental cells in toxicology studies: a promising tool to reveal pregnancy disorders Anat Cell Biol. 2024 Mar 31;54 (1):83-92. doi: 10.5115/acb.20.234. Authors Elodie Olivier 1 , Anaïs Wakx 1 , Sophie Fouyet 1 , Mélody Dutot 1 2 , … " - Jeg-3 cell line

Jeg-3 cell line

HLA-G gene editing in tumor cell lines as a novel alternative

Web4 mar 2016 · We used the RWV bioreactor to develop a 3D-based cell coculture model that induces high levels of spontaneous fusion of a trophoblast cell line, JEG-3 cells. JEG-3 cells cultured in this system exhibit high rates of syncytia formation, which correlates to increased brush border formation and secretion of placental hormones. Web1 gen 2024 · JEG-3 is another independent human choriocarcinoma cell line reported first in 1971 [9]. It is mostly considered a model for invasive trophoblast cells [ 6 ]. JEG-3 …

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Web29 nov 2024 · The BeWo cell line is used to study placental function, since it forms syncytium and secretes hormones after treatment with cAMP or forskolin. In contrast, the … Web13 nov 2013 · Line Spacing: Column ... MCF7 and HepG2 cells were transiently transfected with 3 μg HIS-FLAG-tagged WT Nr5a1 or Nr5a1 in which lysine 194 (K194R), glutamic acid 199 (E199A), or both lysine 194 and glutamic acid 199 (K194RE199A) were mutated to arginine or alanine.

Web10 nov 2006 · The JEG-3 cell line are human choriocarcinoma cells derived from malignant placental tissues. As in placenta, JEG-3 cells endogenously express aromatase activity … Web15 mar 2024 · After electroporation and drug selection, a successful HLA-G knockout JEG-3 cell line (JEG-3 KO cells) was established, and its efficacy was first validated by PCR and western blotting . Additional functional validation was performed on the well-established functions of HLA-G, including the inhibition of natural killer cell cytotoxicity and the …

WebAn understanding of the interactions between immune cells and trophoblast cells, as well as choriocarcinoma cells, are of extreme importance in reproductive immunology and … Web4 mar 2024 · In this system, the trophoblast-derived JEG-3 cell line is co-cultured with human brain microvascular endothelial cells attached to microcarrier beads in a rotating bioreactor. Here, we report that 3D culture of JEG-3/HBMEC spheroids promote JEG-3 cells differentiation revealed by the formation of syncytia and production of β human …

Web31 mar 2024 · JEG-3 cells prepared in our incubation conditions were renamed JEG-Tox cells. As placental alterations are mainly triggered by uncontrolled apoptosis, we second …

JEG-3 is a hypertriploid, clonally-derived, human cell line with epithelial morphology that was isolated from the Woods strain of the Erwin-Turner tumor. This cell line is tumorigenic, capable of forming malignant tumors consistent with choriocarcionoma. Genes expressed: human chorionic gonadotropin (hCG); human chorionic somatomammotropin ... chest disease associates merrillvilleWebWe measured the basal levels of phosphorylated ATR kinase in SW1353, CS-1, 5-8F, and JEG-3 cells. The results showed that the chondrosarcoma CS-1 cell line and SW1353 cells expressed very low levels of phosphorylated ATR at baseline. As experimental controls, 5-8F and JEG-3 expressed moderate levels of phosphorylated ATR . chest discomfort when taking a deep breathWeb1 mag 2016 · JEG-3 Trophoblast Cells Producing Human Chorionic Gonadotropin Promote Conversion of Human CD4+FOXP3− T Cells into CD4+FOXP3+ Regulatory T Cells and Foster T Cell Suppressive Activity1 Biology of Reproduction … good mp3 players ukWeb12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC on JEG-3 edited cells. chest discomfort while walkingWeb"OUMS-36T-2F Cell;OUMS-36T-2F细胞系(STR鉴定图谱) GD-C00513270S 背景信息：详见相关文献介绍产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支) 目前，跟公司签订长期战略合作伙伴的科研单位有山西中医学院、福建医科大 good mpg cars hybridWeb10 nov 2006 · The JEG-3 cell line are human choriocarcinoma cells derived from malignant placental tissues. As in placenta, JEG-3 cells endogenously express aromatase activity at detectable levels (Bahn et al., 1981) and the cyp19 gene expression is under the control of a distal promoter I.1 located adjacently upstream of exon I.1 in the gene (Means et al ... chest disease associatesWebJeg 3 Choriocarcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > ATCC > choriocarcinoma cell line jeg 3. chest disease name