Flp-in 293 cells
WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. These cells contain a single stably integrated FRT site at a trans WebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ...
Flp-in 293 cells
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WebNov 1, 2010 · Wild-type and mutant BCRP-expressing HEK-293 or Flp-In-293 cells and the vector control cells were plated in four-chamber polysterene vessels on glass slides (Falcon; BD Biosciences, Bedford, MA) at ∼2 × 10 5 cells/well. Cells were grown to 80% confluence and washed twice with PBS at room temperature. WebThe cell line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA. A portion of human adenovirus 5, nucleotides (nts) 1-4344, is integrated into chromosome 19 of the HEK cells. Human adenovirus 5 causes a range of respiratory illnesses. HEK 293 cell lines are commonly used in cancer research.
WebApr 1, 2016 · Symbols and abbreviations are as described in Fig. 1. (E) Flp-In-293 cells were transiently transfected with the indicated GPR37 constructs for 24 h, and the anti-Myc-antibody-labelled full-length receptors were analysed by confocal microscopy as … WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In …
WebE , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA … WebNov 8, 2016 · The FH-AKAP95 and FH-AKAP95 (101–692) cell lines were made by co-transfecting Flp-In-293 cells (f293 cells, Invitrogen) with pOG44 plasmid and either an FH-AKAP95-pcDNA5 or an FH-AKAP95 (101 ...
WebArticle Snippet: Flp-In T-Rex Human Embryonic Kidney 293 (HEK293) cells were purchased from ThermoFisher Scientific, while the parental HEK293 cell line was obtained from ATCC (Manassas, VA, USA). Techniques: High Throughput Screening Assay, Promoter Assay, Derivative Assay
WebFlp-In-293 cells and Flp-In-CV-1 cells are generally amenable to transfection using standard methods including calcium phosphate precipitation (Chen and Okayama, 1987; Wigler et al., 1977), lipid-mediated transfection (Felgner et al., 1989; Felgner and … rovi pharma industrial services saWebE , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. rovio world questWebRNA-seq of the NE cells homozygous for W1282X showed that CFTR transcript level was reduced to 1.7% of WT (p-value: 4.6e-3). Negligible truncated CFTR protein was generated by Flp-In 293 cells stably expressing the W1282X EMG even though CFTR transcript was well above levels observed in the parents and proband. rovipak packaging solutions ltdWebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In … rovipharm medicine syringesWebInvitrogen™ Flp-In™ T-REx™ 293 Cell Line. Cells contain single stably integrated FRT site at transcriptionally active genomic locus. Includes: 1 x 10 7 cells are supplied frozen in 1mL of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. rovi products incWebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture … rovipharm treffort cuisiatWebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In-293 cell line was created by transfecting the parent cell ... rovira foods inc